Agroecological transition corresponds to a systemic transformation consisting in the ecologisation of agriculture and food. It concerns multiple stakeholders (farmers, supply chains, natural resource managers, etc.) and is characterised by a deliberate political intention to bring about change. This chapter highlights a set of determinants of agroecological transition at play in transforming the techniques and the values underpinning both agricultural production and food consumption choices – both of which can lead to various new agri-food systems. Based on the literature on transition studies, we focus on several considerations that could help stakeholders to better engage in such a process: (i) transition takes place over time intervals that vary, depending on the analysis scale (the farm or the agri-food system as a whole); (ii) transition is complex, systemic and requires changes of the whole sociotechnical regime; (iii) transition implies strong connections between niche-innovations and the dominant sociotechnical regime; and (iv) changes in values and individuals’ abilities are fundamental drivers. Hence, by focusing on the plurality of factors and stakeholders at work, we unpack the complexity of this transition, and in this way help the stakeholders to design and execute it. To conclude, we examine specific issues around the governance of agroecological transition.