%0 Journal Article %T Multiplexed two-photon microscopy of dynamic biological samples with shaped broadband pulses. %+ Laboratoire d'optique et biosciences (LOB) %+ Ecole Polytechnique %A Pillai, Rajesh, S. %A Boudoux, Caroline %A Labroille, Guillaume %A Olivier, Nicolas %A Veilleux, Israel %A Farge, Emmanuel %A Joffre, Manuel %A Beaurepaire, Emmanuel %< avec comité de lecture %@ 1094-4087 %J Optics Express %I Optical Society of America - OSA Publishing %V 17 %N 15 %P 12741-52 %8 2009-07-20 %D 2009 %R 10.1364/OE.17.012741 %M 19654680 %Z Physics [physics]/Physics [physics]/Optics [physics.optics] %Z Life Sciences [q-bio]/Bioengineering/ImagingJournal articles %X Coherent control can be used to selectively enhance or cancel concurrent multiphoton processes, and has been suggested as a means to achieve nonlinear microscopy of multiple signals. Here we report multiplexed two-photon imaging in vivo with fast pixel rates and micrometer resolution. We control broadband laser pulses with a shaping scheme combining diffraction on an optically-addressed spatial light modulator and a scanning mirror allowing to switch between programmable shapes at kiloHertz rates. Using coherent control of the two-photon excited fluorescence, it was possible to perform selective microscopy of GFP and endogenous fluorescence in developing Drosophila embryos. This study establishes that broadband pulse shaping is a viable means for achieving multiplexed nonlinear imaging of biological tissues. %G English %2 https://polytechnique.hal.science/hal-00818488/document %2 https://polytechnique.hal.science/hal-00818488/file/oe-17-15-12741.pdf %L hal-00818488 %U https://polytechnique.hal.science/hal-00818488 %~ X %~ CNRS %~ X-LOB %~ X-DEP %~ X-DEP-BIO