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              <p>La présente invention concerne un module 1 d'excitation multi-couleurs pour un système d'imagerie multi-photonique 100. Un tel module est utilisé pour imager un échantillon 7 comprenant au moins trois chromophores. Le module 1 comprend : - une première source de laser femtoseconde 2, émettant un premier faisceau d'excitation 20 ; - une deuxième source laser femtoseconde 3 émettant un deuxième faisceau d'excitation 30. Le premier faisceau d'excitation 20 comprend une partie dite « de pompage », cette partie de pompage servant de faisceau de pompe pour exciter de façon synchrone la deuxième source laser 3, et une partie dite « d'excitation ». Une ligne à retard optique 4 est agencée pour superposer spatialement et temporellement le deuxième faisceau d'excitation 30 et la partie d'excitation du premier faisceau d'excitation, de façon à exciter au moins un troisième des chromophores par absorption multi-photons, lesdits photons absorbés provenant des premier et deuxième faisceaux d'excitation 20,30. L'invention concerne également le système complet d'imagerie multi-photonique 100, ainsi qu'un procédé mis en oeuvre dans ce module 1.</p>
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