%0 Journal Article %T Quantitative measurement of permeabilization of living cells by terahertz attenuated total reflection %+ Laboratoire d'Optique et Biosciences (LOB) %A Grognot, Marianne %A Gallot, Guilhem %< avec comité de lecture %@ 0003-6951 %J Applied Physics Letters %I American Institute of Physics %V 107 %N 10 %P 103702 %8 2015 %D 2015 %R 10.1063/1.4930168 %Z Physics [physics]/Physics [physics]/Optics [physics.optics] %Z Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]Journal articles %X Using Attenuated Total Reflection imaging technique in the terahertz domain, we demonstrate non-invasive, non-staining real time measurements of cytoplasm leakage during permeabilization of epithelial cells by saponin. The terahertz signal is mostly sensitive to the intracellular protein concentration in the cells, in a very good agreement with standard bicinchoninic acid protein measurements. It opens the way to in situ real time dynamics of protein content and permeabilization in live cells. %G English %2 https://polytechnique.hal.science/hal-01222843/document %2 https://polytechnique.hal.science/hal-01222843/file/14930168.pdf %L hal-01222843 %U https://polytechnique.hal.science/hal-01222843 %~ X %~ CNRS %~ X-LOB %~ X-DEP %~ X-DEP-BIO %~ PARISTECH %~ INSERM-SACLAY %~ UNIV-PARIS-SACLAY