Tunable biomimetic materials elaborated by ice templating and self-assembly of collagen for tubular tissue engineering - Matériaux et Biolologie Access content directly
Preprints, Working Papers, ... Year : 2023

Tunable biomimetic materials elaborated by ice templating and self-assembly of collagen for tubular tissue engineering

Isabelle Martinier
Florian Fage
Alshaba Kakar
  • Function : Author
Alessia Castagnino
  • Function : Author
Emeline Saindoy
  • Function : Author
Joni Frederick
Ilaria Onorati
Valérie Besnard
Abdul I Barakat
  • Function : Author
Nicolas Dard
Emmanuel Martinod
Carole Planes
Léa Trichet
Francisco M Fernandes

Abstract

Synthetic tubular grafts currently used in clinical context fail frequently, and the expectations that biomimetic materials could tackle these limitations are high. However, developing tubular materials presenting structural, compositional and functional properties close to those of native tissues remains an unmet challenge. Here we describe a combination of ice templating and topotactic fibrillogenesis of type I collagen, the main component of tissues’ extracellular matrix, yielding highly concentrated yet porous tubular collagen materials with controlled hierarchical architecture at multiple length scales, the hallmark of native tissues’ organization. By modulating the thermal conductivity of the cylindrical molds, we tune the macroscopic porosity defined by ice. Coupling the aforementioned porosity patterns with two different fibrillogenesis routes results in a new family of materials whose textural features and the supramolecular arrangement of type I collagen are achieved. The resulting materials present hierarchical elastic properties and are successfully colonized by human endothelial cells and alveolar epithelial cells on the luminal side, and by human mesenchymal stem cells on the external side. The results reported here demonstrate the relevance of the proposed straightforward protocol, likely to be adapted for larger graft sizes, to address ever-growing clinical needs such as peripheral arterial disease or tracheal and bronchial reconstructions.
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hal-04295301 , version 1 (20-11-2023)

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Isabelle Martinier, Florian Fage, Alshaba Kakar, Alessia Castagnino, Emeline Saindoy, et al.. Tunable biomimetic materials elaborated by ice templating and self-assembly of collagen for tubular tissue engineering. 2023. ⟨hal-04295301⟩
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