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Journal Articles Nature Methods Year : 2011

Deep and fast live imaging with two-photon scanned light-sheet microscopy

T.V. Truong
  • Function : Author
D.S. Koos
  • Function : Author
J.M. Choi
  • Function : Author
S.E. Fraser
  • Function : Author

Abstract

We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large biological samples. Live imaging of fruit fly and zebrafish embryos confirmed that the technique can be used to image up to twice deeper than with one-photon light-sheet microscopy and more than ten times faster than with point-scanning two-photon microscopy without compromising normal biology. Cop. 2011 Nature America, Inc. All rights reserved.
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hal-00803780 , version 1 (25-03-2013)

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T.V. Truong, Willy Supatto, D.S. Koos, J.M. Choi, S.E. Fraser. Deep and fast live imaging with two-photon scanned light-sheet microscopy. Nature Methods, 2011, 8 (9), pp.757. ⟨10.1038/nmeth.1652⟩. ⟨hal-00803780⟩
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