Large-scale live imaging of adult neural stem cells in their endogenous niche

Live imaging of adult neural stem cells (aNSCs) in vivo is a technical challenge in the vertebrate brain. Here, we achieve long-term imaging of the adult zebrafish telencephalic neurogenic niche and track a population of >1000 aNSCs over weeks, by taking advantage of fish transparency at near-infrared wavelengths and of intrinsic multiphoton landmarks. This methodology enables us to describe the frequency, distribution and modes of aNSCs divisions across the entire germinal zone of the adult pallium, and to highlight regional differences in these parameters.

Domaines

Optique [physics.optics] Imagerie Neurobiologie

Fichier principal

3592.full.pdf (4.55 Mo)

2015 Dev Large-scale live imaging of adult NSCs - Supp.pdf (2.44 Mo)

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Denis Roura : Connectez-vous pour contacter le contributeur

https://polytechnique.hal.science/hal-01221855

Soumis le : jeudi 29 octobre 2015-16:05:27

Dernière modification le : jeudi 1 août 2024-13:48:06

Archivage à long terme le : vendredi 28 avril 2017-05:13:54

Dates et versions

hal-01221855 , version 1 (29-10-2015)

Identifiants

HAL Id : hal-01221855 , version 1
DOI : 10.1242/dev.123018
PUBMED : 26395477
PUBMEDCENTRAL : PMC4631764

Citer

Nicolas Dray, Sébastien Bedu, Nelly Vuillemin, Alessandro Alunni, Marion Coolen, et al.. Large-scale live imaging of adult neural stem cells in their endogenous niche. Development (Cambridge, England), 2015, 142 (20), pp.3592-3600. ⟨10.1242/dev.123018⟩. ⟨hal-01221855⟩

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