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Journal Articles Nature Communications Year : 2019

Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy

Anatole Chessel
Karine Loulier
Willy Supatto
Jean Livet
  • Function : Correspondent author
  • PersonId : 1046341

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Large-scale microscopy approaches are transforming brain imaging, but currently lack efficient multicolor contrast modalities. We introduce chromatic multiphoton serial (ChroMS) microscopy, a method integrating one-shot multicolor multiphoton excitation through wavelength mixing and serial block-face image acquisition. This approach provides organ-scale micrometric imaging of spectrally distinct fluorescent proteins and label-free nonlinear signals with constant micrometer-scale resolution and sub-micron channel registration over the entire imaged volume. We demonstrate tridimensional (3D) multicolor imaging over several cubic millimeters as well as brain-wide serial 2D multichannel imaging. We illustrate the strengths of this method through color-based 3D analysis of astrocyte morphology and contacts in the mouse cerebral cortex, tracing of individual pyramidal neurons within densely Brainbow-labeled tissue, and multiplexed whole-brain mapping of axonal projections labeled with spectrally distinct tracers. ChroMS will be an asset for multiscale and system-level studies in neuroscience and beyond.
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hal-02114066 , version 1 (20-11-2019)




Lamiae Abdeladim, Katherine S. Matho, Solène Clavreul, Pierre Mahou, Jean-Marc Sintes, et al.. Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy. Nature Communications, 2019, 10 (1), pp.1662. ⟨10.1038/s41467-019-09552-9⟩. ⟨hal-02114066⟩
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