Determination of collagen fibril size via absolute measurements of second-harmonic generation signals. - École polytechnique Access content directly
Journal Articles Nature Communications Year : 2014

Determination of collagen fibril size via absolute measurements of second-harmonic generation signals.

Abstract

The quantification of collagen fibril size is a major issue for the investigation of pathological disorders associated with structural defects of the extracellular matrix. Second-harmonic generation microscopy is a powerful technique to characterize the macromolecular organization of collagen in unstained biological tissues. Nevertheless, due to the complex coherent building of this nonlinear optical signal, it has never been used to measure fibril diameter so far. Here we report absolute measurements of second-harmonic signals from isolated fibrils down to 30 nm diameter, via implementation of correlative second-harmonic-electron microscopy. Moreover, using analytical and numerical calculations, we demonstrate that the high sensitivity of this technique originates from the parallel alignment of collagen triple helices within fibrils and the subsequent constructive interferences of second-harmonic radiations. Finally, we use these absolute measurements as a calibration for ex vivo quantification of fibril diameter in the Descemet's membrane of a diabetic rat cornea.
Fichier principal
Vignette du fichier
2014 post-print NatCom.pdf (1.25 Mo) Télécharger le fichier
Origin Files produced by the author(s)
Loading...

Dates and versions

hal-01079871 , version 1 (14-11-2014)

Identifiers

Cite

Stéphane Bancelin, Carole Aimé, Ivan Gusachenko, Laura Kowalczuk, Gaël Latour, et al.. Determination of collagen fibril size via absolute measurements of second-harmonic generation signals.. Nature Communications, 2014, septembre (5), pp.4920. ⟨10.1038/ncomms5920⟩. ⟨hal-01079871⟩
863 View
592 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More